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Genotypes were scored by analyzing the electropherograms from the fragment analysis using GeneMarker version 1.95 (SoftGenetics, State College, Pennsylvania, USA).
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For the study, Hampikian and U.K. psychologist Itiel Dror asked 17 analysts at a reputable U.S. lab to interpret the electropherograms from the case, without specifying their origin.
The electropherograms from the 1,172 failed reads were evaluated and subsequently categorized into failure mode categories.
The electropherograms from the Bioanalyzer showed a smear of degraded RNA in a size range of 100 200 bp (data not shown).
The electropherograms from both reactions were strong and gave an unambiguous signal corresponding to the expected sequence in each case (Fig. 6).
The electropherogram from this reaction displayed strong signal strength and the expected sequence (Fig. 3B).
The methylation beta value from the Illumina methylation arrays was compared with the peak heights of the sequencing electropherograms from bisulfite sequencing of the genes where the CpG site analyzed by the methylation array was inside of the PCR fragment analyzed by BSP (TGFBI, PRKCDBP, DUSP23 and COL1A2).
The data were analyzed using the GeneScan software to obtain the electropherograms and polymorphisms were analyzed with Genotyper DNA Fragment Analysis software.
The electropherograms were analyzed in Genemapper 3.7 (Applied Biosystems).
The electropherograms were analyzed using the pipeline ParPEST developed at the University of Naples [ 5].
The electropherograms were analyzed with Mutation Surveyor software (Softgenetics, State College, PA) to determine the percentage of methylation for every cytosine residue.
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