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The TCR specificity was then examined by analyzing the binding of peptide HLA-A2/tetramer in an ELISA assay.
After analyzing the binding of an HSPB6-derived phosphopeptide to 14-3-3 using isothermal calorimetry and X-ray crystallography, we have determined the crystal structure of the complete assembly consisting of the 14-3-3 14-3-3 14-3-3l-length HSPB6 dimer and further characterized this complex in solution using fluorescence spectroscopy, small-angle X-ray scattering, and limited proteolysis.
IC50 plots were generated by analyzing the binding of EBNA1 or Zta with inhibitor compounds ranging from 833 to 7 uM final concentrations following two fold dilutions (specifically 833, 417, 208, 104, 52, 26, 13, 7, and 0 µM).
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We analyzed the binding of its nucleotide substrates by fluorometric methods.
Therefore in this study, we employed a series of computational strategies to analyze the binding of antiviral drug, Boceprevir to HCV NS3/4A protease mutants.
Differential scanning calorimetry, UV/visible spectroscopy, and molecular modeling were used to analyze the binding of 15 azo molecules to avidin and AVR4.
Here we present a new strategy to analyze the binding of multiprotein CSL complexes to DNA.
In addition to cell death we have also analyzed the binding of C4BP to H9C2 cells cultured in hypoxic conditions.
In order to validate these findings, we analyzed the binding of C-terminal HCN2 constructs (HCN2-CT and HCN2-CT-S641A HCN2-CT-S641A HCN2-CT-S641A
To better understand how TRF2 performs this function in the context of chromatin, we analyzed the binding of TRF2 to fibers of nucleosomal arrays in comparison to histone-free DNA.
To determine the uptake of the M-hMPV protein by moDCs, we analyzed the binding of M-hMPV labeled with fluorescein to moDCs and to MDMs by flow cytometry.
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