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After 15 min, samples were taken for analyzing the activation of crude enzyme liquid by HPLC.
We therefore established two epiSC reporter lines to examine reprogramming by monitoring the status of X reactivation and by analyzing the activation of Oct4 DE in response to germline factors (see also later).
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Moreover, we analyzed the activation of some activator protein (AP -1 trAP -1iptranscriptionomponents.
In order to examine whether MyD88 has a direct role in the macrophage response to Mp, we analyzed the activation of NFκB, one of the major downstream transcription factors activated by TLR-MyD88 signaling, in WT or MyD88−/− BMM treated in vitro with EYFP-Mp.
To determine how LRRK2 may affect the production of inflammatory cytokines in macrophages in response to MDP, we analyzed the activation of the NF-κB and MAPK pathways, which are known to be responsible for cytokine induction downstream of Nod2-Rip2.
In addition, we have used the newly engineered APPSL/presenilin 1 knock-in transgenic mice, which display a severe neuronal loss in hippocampal regions, to analyze the activation of double-stranded RNA-dependent protein kinase.
In the same p53-induced transcriptional setting, we analyzed the activation of p53 during long periods and its correlation with the appearance of senescence.
To analyze the activation of DCs and NK cells in vivo by Pam2 lipopeptides, 10 nmol of Pam2 lipopeptides was subcutaneously (s.c).
First, we analyzed the activation of cultured human aortic endothelial cells (HAEC) exposed to supernatant from monocytes infected for 16 h.
To define the cellular response of SKOV-3 cells upon Fe-SP treatment we analyzed the activation of caspases characteristic for induction of apoptosis as well as the inactivation of PARP-1 by immunoblotting.
Since the duration of bystander medium conditioning was reported to be one of the key factors affecting the bystander signaling after IR exposures [24], we analyzed the activation of DDR in bystander hMSC with 24 hr medium conditioning (Fig. 2).
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