Mass spectrometry was used to analyze gaseous oxidation products and solid products were identified by the XRD technique.
Thus, the absorbance at this wavelength (A421) can be utilized to quantitatively analyze the oxidation product and evaluate the DNAzyme functions of different G-quadruplex aptamers.
These catalysts were characterized using X-ray diffraction (XRD), UV vis diffuse reflectance (DRUV vis), N2 sorptiometry, Raman spectroscopy, transmission electron microscopy (TEM) and GC MS technique, which used for analyzing the photo-oxidation products.
SA oxidation products were analyzed by gas chromatography mass spectrometry (GC MS), gas chromatography (GC) and CO2 analyzer, and SA was oxidized selectively to ODDA without producing CO2 and intermediates like alcohols, aldehydes and ketones.
Selected samples were further analyzed for identification of adenine oxidation products by HPLC-MS, utilizing electrospray ionization and a time-of-flight (TOF) mass detector (MicroMass LCT™).
The oxidation products were analyzed by HPLC using a Shimadzu LC-600 liquid chromatograph, Hamilton PRP-1 columnlumn (250×4.1 mm dimensions, 7 µm particle size), and Shimadzu SPD-6AV UV detector.
For analysis of complex biological samples, TLC-MS is able to separate and analyze the components and their oxidation products, even those that are present at the same R f value.
The hydrolyzed oxidation products were then analyzed with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
The cellulose oxidation products were also analyzed with an HPAEC-PAD system according to the method described by Rantanen et al. [ 50] for analysis of oligosaccharides.
Oxidation products and by-products were analyzed both on-line by GC and off-line by GC-MS, and slight difference was observed for these two chlorinated benzenes.
These data were used to purposefully generate the corresponding oxidation products electrochemically which were often analyzed by spectroscopic methods during their nascency.
