We addressed this hypothesis indirectly, by using cytokine antibody arrays and ELISA assays to analyze conditioned media from the R2-T1AS/G2B-10A and the R2-T1AS/G2B-10A(PFA) co-cultures, in which cells were plated in suspension at a high density, thus allowing them to aggregate and form direct cell-cell contacts.
We analyzed the incubation media from the culture wells using HPLC with electrochemical detection in order to provide an indication of the DA levels at equilibrium after 15+days in culture.
Cytokine levels were analyzed in conditioned media from astrocytes, mono-, and cocultures using the (RayBiotech, Norcross, GA) Human Cytokine Antibody Array G Series 5 according to the manufacturer's instructions with two subarrays per sample.
This study contributes to this literature by content analyzing national media discourse on motor vehicle emissions from 2000 to 2008.
This surveillance includes analyzing more media sources and regularly requesting information from the WHO network about outbreak events.
4. Analyze media coverage of the 2004 election from three different media sources.
Shortly after, there was a request by an intelligence agency to help analyze social media posts from a volatile region of the world.
To test if bladder cancer cells also secrete DEK protein, we analyzed conditioned media obtained from bladder cancer cell line 5637.
Olson's research focuses on inventing and analyzing new forms of media, from gaming experiences to interactive narratives.
Clearly, information about time is not always available, but when analyzing posts from social media (e.g., Twitter, web forums, etc)., such information can often be extracted.
We further analyzed day 3 conditioned media from PBMCs incubated with Pam3CSK4, PG-LPS, LTA, PGN and LPS for the presence of Th1/Th2/Th17 cytokines.
