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The total cellular activity was corrected for non-specific binding (10 μM PMPA).
Muscle uptake of 18F-MK-9470 was used as reference for non-specific binding.
In contrast, its CHI IAM) value of 58 clearly indicated a high tendency for non-specific binding.
The values are corrected for non-specific binding determined by co-incubation with 1 μM natGa-labelled peptide.
The total cellular activity was corrected for non-specific binding in the presence of 100 μM AMD3100.
This also controlled for non-specific binding of DiI-LDL.
For each animal, three samples were used for total binding and one for non-specific binding.
A control selection was performed to control for non-specific binding to the antibody-bead matrix.
Images were corrected for non-specific binding of secondary antibodies, as detailed in Methods.
For non-specific binding residue prediction, there are 3831 binding residues and 21066 non-binding residues.
Residues colored by red for sequence-specific binding residues, blue for non-specific binding residues, and purple for both sequence-specific and non-specific binding residues.
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