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Briefly, we verified the genotyping quality by three steps: 1) a number of samples were genotyped in both genomic and amplified DNA; 2) inheritance of alleles between parents and children was checked using FBAT (http://biostat.harvard.edu); 3) genotype data were analyzed for deviations from Hardy-Weinberg equilibrium using χ2 statistics.
The genotype distribution of each tSNP was analyzed for deviations from the Hardy-Weinberg equilibrium using χ2 analyses.
The genotype data were analyzed for deviations from Hardy-Weinberg equilibrium by the Haploview version 4.1 software [ 24].
The data were first analyzed for deviations from the assumptions of ANOVA, and outliers were identified via box plots.
Allele frequencies were calculated and analyzed for deviations from Hardy Weinberg equilibrium (HWE) using χ-tests with 1 degree of freedom.
For the analysis of the short-term physiological response, physiological parameters were analyzed for deviations from zero over the course of the response window separately for both piloerection conditions by means of one-sample t-tests.
For the analysis of the general physiological response, first, physiological parameters were analyzed for deviations from zero (significant increase or decrease) separately for both piloerection conditions by means of one-sample t-tests.
For statistical analysis, all experiments were analyzed for deviation from additivity with an n-factor analysis of variance with an interaction term, n being the number of components tested in combination.
To ensure central fixation after presentation of the spatial cue, the period between cue and target was analyzed for gaze deviations from the center.
The data was statistically analyzed for standard deviation and error using Sigma Plot software (2004).
For normal distribution, the mean and standard of deviation values were analyzed for significant difference between the two groups using Student's t-test.
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