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LC MS/MS analyzes were performed using a CTC PAL autosampler (Thermo) with a MS gradient pump (Thermo) interfaced to a TSQ Quantum Access triple quadrupole mass spectrometer (Thermo) equipped with a heated ESI probe.
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To analyze prognostic factors, univariate and multivariate analyses were performed using a Cox proportional hazards model.
Analyzes were performed using QSR NVivo software (version 9.0) and supported with a coding manual as well as Microsoft Word.
All the statistical analyzes were performed using SPSS v. 18 (IBM, Chicago, IL, USA), and a two-tailed P<0.05 was considered as the significance limit for each of the different tests.
Gene expression analyzes were performed using 3 arrays, representing 3 biological replicates with one replicate done in a dye-swap manner to test for dye-based bias.
All statistical analyzes were performed using the IBM SPSS Statistics 21.0 (SPSS Inc. IBM, Armonk, NY) software with 2-sided tests, with a P-value of <0.05 considered as statistically significant.
To exclude an influence of different trap heights, comparative analyzes were performed using the same statistics but only for trappings at the same height.
All analyzes were performed using flow cytometry.
All the statistical analyzes were performed using the SPSS Base 19.0 for Windows (SPSS Inc., USA).
All statistical analyzes were performed using R.
All biochemical analyzes were performed using commercially available kits, according to the Manufacturer's protocols.
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CEO of Professional Science Editing for Scientists @ prosciediting.com