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In all the following analyzes we use the set of mutations classified as high-confidence mutations in Tuberculosis Drug Resistance Mutation Database (TBDReaMDB) as our gold standard [ 5].
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Since Q is susceptible to inaccuracy due to the small number of studies analyzed we used the I2 index to quantify the degree of heterogeneity attributed to between-studies variation.
In order to analyze the data, we used the SPSS (Statistical Package for the Social Sciences) version 17.0 software for Windows.
To analyze the data, we used the ΔCp value that provided by the Lightcycler 480 system analysis software.
To analyze calcium deposition, we used the Alizarin Red S Staining.
To further analyze the FRAP data, we used the Virtual Cell software in simulation of NS1 dynamics.
For analyzing differential fluorescence data we used the MetaSystems ISIS 3 software.
To analyze the stability, we use only the energy integral.
Of the TCGA samples analyzed by Noushmehr et al. [33], 88 overlapped with the samples that we analyzed using the publicly available dataset.
We collected data from at least three different experiments and we analyzed them using the 7000 v1.1 SDS instrument software (Applied Biosystems).
We analyzed PCA using the R function "prcomp" and used PC1, PC2 and PC3 for the PCA sharing plot.
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