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Twenty micrograms of fragmented biotinylated cRNA were used to prepare the hybridization cocktail and subsequently hybridized for sixteen hours to the Human Genome U133A plus 2.0 microarrays, which analyzes the expression level of over 47000 transcripts and variants.
This product analyzes the expression level for over 135,000 transcripts and variants, including 44049 well-substantiated human genes.
Through such a panel the user analyzes the expression level of each single exon in each sample.
This product analyzes the expression level for over 39,000 transcripts and variants, including over 34,000 well-substantiated mouse genes.
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To further evaluate our DGE data, we analyzed the expression level of vitellogenin which is an egg yolk gene highly expressed in the adult female [ 30].
We further analyzed the expression level of this gene in the brain of Prnp-knockout mice expressing or not the NFH-Cre transgene.
Gene Ontology (GO) functional annotation analysis was used to analyze the expression level of all identified proteins.
Furthermore, we analyzed the expression level of several genes related to grain size.
Moreover, we also analyzed the expression level of a gene YFR057W near the telomere of chromosome VI (Fig. 1G).
Both RT-PCR and qRT-PCR were used to analyze the expression level of REL2/Os10g0562700 in WT and rel2 mutant.
Finally, we analyzed the expression level of hormone receptor genes in granulosa cells and their relationship with follicular atresia.
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