Neonatal cardiac myocytes (50 000–100 000 cells/well) or human iPS-derived cardiac myocytes (60 000/were) were plated in 24-well Seahorse analyzer plates.
Briefly, 0.45 μl of digested protein solution was mixed in 0.45 μl of α-cyano-4-hydroxycinnamic acid solution on matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) 4800 proteomics analyzer targeted plate.
Briefly, 7 × 10 cells/well IGROV1-R10 or 3.5 × 10 cells/well SKOV3 were plated in 96-well E-Plate View and placed onto the Real-Time Cell Analyzer multi-plate located inside a tissue culture incubator.
Real-time growth curves monitoring was performed with the Real-Time Cell Analyzer multi-plate instrument, using the xCELLigence System (ACEA, Ozyme, Saint Quentin en Yvelines, France).
Cell growth was monitored in real time using a Real-Time Cell Analyzer Dual-Plate (RTCA DP) instrument (Roche Applied Science, P.O. Box 50414, 9115 Hague Road, Indianapolis, IN 46250-0414), placed in a humidified incubator maintained at a 5% CO2 and 37 °C.
Live cells were analyzed using an Accuri C6 cell analyzer with Intellicyte plate sampler attachment.
Briefly, 3 × 10 cells were re-suspended in RPMI media and added to each poly- D-lysine-coated well in an XF24 Analyzer 24-well plate.
Respiratory and glycolytic rates in four wild-type and four knockout MEF cell lines were measured with the XF Analyzer 96-well plate reader.
Further improvements in sensitivity and detection limits can undoubtedly be implemented by mating the interface with a mass spectrometer equipped with a more advanced ion optics, mass analyzer, and multichannel plate ion detector.
This review focuses on the clinical utility of two representative instruments: impact cone and plate analyzer (DiaMed, Cressier, Switzerland) [ 2, 18, 19] and thrombelastography (TEG) (Hemoscope, Niles, IL, USA) [ 7– 10, 21, 22] in more detail, and the others are referred to below in the section of monitoring antiplatelet therapy.
