Outcomes of the performed study are validated by analyzing the posture of 150 ants when loitering on vertical surfaces.
Five out of seven analyzed CNVRs were validated by real time quantitative PCR, some of which coincide with well known examples of CNVs conserved across mammalian species.
Even though there was a variation in fold expression of DEG genes between RNA-seq and qRT-PCR; more than 90%% of analyzed genes were validated by qRT-PCR analysis.
The methods were validated by analyzing IAEA-140/TM, Fucus – Sea Plant Homogenate – and NIES-09, Sargasso, certified reference materials.
The accuracy of the microarray results were validated by analyzing 14 differentially expressed genes by qRT-PCR using the same samples of total RNA.
Therefore, the overall proteins involved in the DPN were validated by analyzing its significance by a random sampling method.
Microarray data were validated by analyzing the expression profile at 0, 2 and 24 h excess light stress.
The findings were validated by analyzing the domain architecture, the subsistence of shared synteny and expression in different tissues of Arabidopsis and tomato, which facilitates the correlation of orthologues and the search for functional connotation.
Due to the potential complication of nonsense mutations and subsequent nonsense-mediated decay of RB1 mRNA, our results were validated by analyzing exons 8, 10, 11, 14, 15, 17, 18 and 23 on genomic DNA from 28 out of 38 patients in the core cohort from whom DNA was available.
Most (12 out of 15, 80%) of the analyzed cases of differentially retained introns were validated by RT-PCR.
Selected proteins as NPM1, RKIP, HSP90B1 and NCL were also differentially expressed among the analyzed subgroups and these levels of expression were validated by western blotting of pooled samples (Fig. 2).
