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Additionally, either the user can elect to use the R console or a prompt window to select the working directory (WD), the directory where the ADAPT 5 model to be analyzed was run.

Given these priors and the data, MCMC iterations for each data set analyzed are run until the estimates for the posterior distributions for the β's and the σ's are stabilized.

Each sample analyzed in this study was run in duplicate and consensus data compiled.

Data were processed on SPSS 17, and statistical analyzes were run.

Data was then exported to Stata 10.0 where frequencies and statistical analyzes were run.

Five biological replicates of each sample were analyzed, and each sample was run in triplicate.

Standard curves for all analyzed genes were run on each plate, by using serial diluted cDNA to normalize the runs.

Each sample was run and analyzed in triplicate.

Real-time qPCR was run and analyzed with a Biorad IQ5 cycler (Biorad).

The qRT-PCR was run and analyzed using the Lightcycler 480 (Roche Diagnostics).

Quantitative real-time PCR was performed using Quanta Perfecta SYBR-Green with ROX Master Mix and was run and analyzed as previously described (Doe et al., 2011).