The effect of Aβ40 on the quantification of Aβ42 was evaluated using LQC and HQC CSF solutions analyzed in the presence of either Aβ40 at 20 %, 50% and 1000 % of the assay ULOQ or hemolyzed blood spiked at 1 in 10, 1 in 100 and 1 in 1,000 (10, 1, 0.1 % blood contamination, respectively).
The VEGF positive cell area in five randomly selected fields was evaluated using NIH digital-image analyzing software, Image J 1.37v, (NIH, Bethesda, MD).
Data were analyzed using iQ5 Optical System Software Version 2.0 (Bio-Rad Laboratories, Inc).. Statistical significance was evaluated using GraphPad Prism 4.0.
The detection performance of the primers was evaluated using ROC curve analyzes.
The fluorescence intensity was evaluated using a BD FACSCalibur® flow cytometer and was analyzed using FlowJo® Cytometry Analysis software (Tree Star Inc., Ashland, OR).
The survival curve was evaluated using the Kaplan-Meier method, and statistical differences were analyzed using the log-rank test.
Locomotor recovery was evaluated using BBB score.
Depression was evaluated using the Center for Epidemiological Studies Depression Scale CES-D Scale CES-D
Binding was evaluated using flow cytometry.
Lipid peroxidation was evaluated using PAR immunohistochemistry.
