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The microscopic density of each CCA structure analyzed was determined using IPLab 4.0 imaging software (Scanalytics).

The number of binding sites in the S. cerevisiae genomes analyzed was determined using PATMAN software [ 52] and allowing from 0 to 3 variable positions in the predicted sequence.

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The volume of the analyzed region was determined using Cavalieri's principle with NIH ImageJ software [53].

To establish absolute CEC counts, the volume of blood analyzed was determined by using the lymphocyte or monocyte counts obtained from the patients' differential.

Densitometry measurements were analyzed and statistical significance was determined using independent Student's t-test.

Six animals per group were analyzed and statistical significance was determined using Student t-test.

When multiple groups were being analyzed the statistical difference was determined using the one-way analysis of variance (ANOVA).

Data was analyzed in Excel and statistical significance was determined using a one-tailed student t test, with unequal variance.

Subsequently, genotype profiles were analyzed using dChip [ 23] and LOH was determined using a panel of 60 normal genotypes from the HapMap dataset [ 24] as provided by dChip, as matching blood lymphoblast profiles were not available.

Data were analyzed with GraphPad Prism 6. Statistical significance was determined using the unpaired two-tailed Student's t-test.

For quantitative analysis of the Golgi/TGN fragmentation, the same threshold was applied to all images, and the number of Golgi-derived particles per cell was determined using the 'Analyze Particles' function of the ImageJ software, as described elsewhere [44].

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