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To evaluate the comparative efficacy of the assay, identical samples were also analyzed via microscopy and Sanger sequencing.
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The microstructure strength relationships were analyzed via electron microscopy and X-ray computed tomography (CT).
Three human cell lines, HeLa cell, HEp-2 cells, and U937 cells, were treated with OMVs acquired from A. baumannii ATCC 19606T for 24 h and the cellular distribution of AbOmpA, a documented OMV protein, was analyzed via confocal microscopy.
Samples were analyzed via confocal microscopy.
Injected embryos were incubated at 28°C until the indicated stage and analyzed via brightfield microscopy.
All samples were analyzed via fluorescence microscopy to determine the presence, distribution and density of photosynthetic symbionts.
The sections were analyzed via light microscopy, and photomicrographs were taken with an Axiostar Zeiss microscope (Zeiss, Germany).
Cells were fixed, permeabilized, stained with rabbit monoclonal anti-p65 (1 : 300 dilution) and analyzed via confocal microscopy as previously described.
The reaction was stopped by replacing the substrate solution with Na-acetate buffer and the staining was analyzed via light microscopy (Axiovert 25, Zeiss).
Protoplasts transfected with the same constructs but analyzed via fluorescence microscopy displayed uniform fluorescence of the protoplast surfaces (Additional file 4).
Briefly, the cells were incubated for 30 min with regular culture medium supplemented with 2 μM calcein AM and 4 μM EthD-1 and analyzed via fluorescent microscopy (Leica DMI6000, Buffalo Grove, IL).
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