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Immunofluorescences in IMR90 cells were analyzed using the high-throughput InCell Analyzer 1000 (GE Healthcare) following manufacturer's instructions.

The morphology of the nanoparticles was analyzed using the high-resolution images obtained with a JEOL 3010 transmission electron microscope and scanning electron microscope.

The samples were first analyzed using the High-Sensitivity DNA Kit for the Bioanalyzer (Agilent Technologies) to detect any primer-dimers and to determine the average molecular weight of each library.

Oxygen consumption was analyzed using the high-resolution oxygraph OROBOROS® (Oroboros Instument, Innsbruck, Austria).

Glucose, xylose, mannose, arabinose, and galactose were analyzed using the high-performance anion-exchange chromatography (HPAEC) ICS-3000 system from Dionex (Sunnyvale, CA, USA) with an electrochemical detector.

BaP DNA adducts in extracted white blood cell DNA were analyzed using the high performance liquid chromatography (HPLC)/fluorescence method of Alexandrov et al. (1992), which detects BaP tetraols.

BaP DNA adducts in extracted white blood cell DNA were analyzed using the high-performance chromatrography (HPLC)/ fluorescence method of Alexandrov et al. (1992), which detects BaP tetraols (Alexandrov et al. 1992; Rojas et al. 1994); the method was modified as described previously (Perera et al. 2004).

BaP DNA adducts in extracted white blood cell DNA were analyzed using the high-performance liquid chromatrography (HPLC)/fluorescence method of Alexandrov et al. (1992), which detects BaP tetraols (Alexandrov et al. 1992; Rojas et al. 1994), modified as described (Perera et al. 2004b).

These two samples were re-analyzed using the higher density 250K SNP array and clearly showed MYCN amplification.

Plates were analyzed using the ArrayScan high content screening (HCS) system (Cellomics, PA, USA).

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