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The different gene expression patterns were analyzed using the Array Assist software version 2.0 (Stratagene, La Jolla, California, United States) that calculated a robust multi-array average of background-adjusted, normalized, and log-transformed intensity values applying the Robust Multi-Array Average algorithm (RMA).
Purified DNA from each nuclear matrix/scaffold and loop fraction was analyzed using the array containing human chromosomes 14, 15, 16, 17 and 18 (Array 7 of the 8 array set) from the Nimblegen Systems CGAR0150-WHG8 CGH isothermal oligonucleotide array system (Nimblegen Systems, Inc., Madison, WI, USA).
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In addition, 20 non-small cell lung cancer (NSCLC) samples were analyzed using the arrays, and both somatic and germline mutations were found.
Twenty NSCLC samples were analyzed using the arrays, and several well-characterized somatic mutations and germline variants were found.
A case was considered positive when at least 70% of tumor cells showed strong nuclear and/or cytoplasmic staining 18. Tumors positive for p16 were subsequently analyzed using the Linear array HPV Genotyping test (S01710; Roche, Almere, the Netherlands) as well as the Linear array Detection kit (S03373; Roche) to confirm HPV-positive status.
In this study, the genetic background of the improved NILs was analyzed using the SNP array RICE6K, which contains 5,102 SNP and InDel markers.
The samples were acquired on a FACSCanto II (BD Biosciences) and analyzed using the FCAP array software (BD Biosciences).
Breath samples would be taken from a wide population with gastric complaints and analyzed using the sensor array.
Total RNA was extracted and analyzed using the Affymetrix GeneChip Human Gene ST array, which has 28,869 unique genes (or transcripts) which are captured by ~26 probes per gene.
Moreover, various unique cases suffering from cancer are currently being analyzed using the Affymetrix Array 6.0 microarray technology to identify copy number changes and homozygosity stretches.
The GMAP array is not limited to use with the pooled reagents described here - in fact, any screen which uses the yeast deletion barcodes or TRC shRNA sequences as molecular barcodes can be analyzed using the GMAP array.
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