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Blood samples (5 mL) were collected at predetermined times and analyzed using a validated high performance liquid chromatography with fluorescence detection method.
The effect of increased power density on charging coefficient is analyzed using a validated model.
Blood samples (5 mL) were collected at a programmed time and analyzed using a validated with fluorescence detection method.
Salt samples from 24 governorates were collected at wholesaler level (n = 635), retailer level (n = 1440) and household level (n = 1560), and analyzed using a validated iodometric titration method.
Eating behavior was analyzed using a validated Dutch translation of the Three Factor Eating Questionnaire (TFEQ) which measures three components: 'cognitive restraint of eating' (factor 1), 'disinhibition of restraint' (factor 2), and 'subjective feeling of hunger' (factor 3) [21].
PAMS data were analyzed using a validated and published method (5).
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The selected structures of the fusion proteins with different linkers were then validated and analyzed using a Ramachandran plot assessment [356].
Estrogens were analyzed using a highly sensitive gas chromatography mass spectrometry (GC/MS) method (validated according to FDA Guidance for industry).
Cortisol concentration was analyzed using a radioimmunoassay (Coat-A-Count; Diagnostics Product Corporation, Los Angeles, CA, USA) validated for caprine plasma.
Maxdiff data was analyzed using a scaled simple count method, which is easy to comprehend and has been previously validated [ 19, 21].
To validate the method, the viability of our corticostriatal organotypic slice cultures was analyzed using a standardized protocol for PI uptake.
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