The phrase "analyzed using a secondary" is correct and usable in written English.
It can be used in contexts where you are describing a method of analysis that involves a secondary source or method.
Example: "The data was analyzed using a secondary approach to validate the findings from the primary research."
Alternatives: "assessed with a secondary" or "evaluated through a secondary".
ORF1 and ORF2 of Drosophila pgc were analyzed using a combination of secondary structure prediction programmes, including PredictProtein (www.predictprotein.org), IUPred (iupred.enzim.hu), GlobPlot 2 (globplot.embl.de) and Agadir [9].
Cultures were fixed in 3.7% paraformaldehyde 30 min RT, immunostained with a polyclonal rabbit anti-human FN antibody followed by goat anti-rabbit Rhodamine labeled IgG secondary (Sigma) and analyzed using a Leica TCS SP5 confocal microscope at 63X.
Samples were paraformaldehyde fixed, permeabilized in 0.2% Triton X-100, incubated with appropriate primary and secondary antibodies, and analyzed using a fluorescence microscope (Leica DM IRBE), as fully detailed in the Supplemental Data.
Continuous secondary efficacy variables used a similar ANCOVA model, and categorical secondary efficacy variables were analyzed using a Cochran Mantel–Haenszel method with stratification by HbA1c values after run-in (<8.0, ≥8.0%) and TZD use (yes or no).
Most secondary endpoints will be analyzed using a Fishers' exact test; endometrium thickness will be analyzed using a student t-test.
Secondary outcomes will be analyzed using a similar strategy, but logistic regression instead of linear regression will be used for these binary outcomes.
The secondary clinical outcome variables were analyzed using a two-level hierarchical random intercepts model.
The secondary structure of the ERRE was analyzed using a combination of computational and experimental approaches.
All categorical variables will be analyzed using a t-test where appropriate, and proportional variables will be analyzed using chi square analysis for secondary outcomes.
The binding of HRP-conjugated ãRabbit IgG (Sigma) secondary antibody was detected using Super Signal West Dura Extended reagent (Pierce) and analyzed using a FluorChem digital camera (Alpha Innotech).
