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Using RSV N as a marker for viral RNPs, we analyzed the colocalization of SBA-488, RSV N, and RSV F in infected cells, where SBA was delivered immediately prior to fixation (Supplementary Fig. 3a).

We then analyzed the colocalization of PI4KIIα, PKD, and insulin.

We then analyzed the colocalization of the BOR1-GFP signal with an early endosome that can be marked with FM4-64.

In heterozygous IL-7 reporter mice, with a gfp gene introduced into one of their Il7 genes [ 20], we analyzed the colocalization of CD8+ memory T cells with stromal cells.

Finally, we offer a slightly different interpretation of the data in Nickell et al, which is fully compatible with our conclusions (C) A. To directly examine the similarity of expression between the H2be:Flag-H2be transgene and the endogenous H2be gene, we analyzed the colocalization of FLAG-H2BE protein and H2be mRNA using combined IF and ISH.

In the present study, we used fluorescence microscopy to analyze the colocalization of RNP and glycoprotein granules over the course of an infection and found that RNPs and glycoprotein vesicles merged as early as 6hpi.

At all ages, images were acquired with a confocal Olympus microscope at 40×magnification, (N.A. = 0,85, field 353×353 µm acquired at 1024×1024 pixels) to analyze the colocalization of antigens and at 20×(N.A. = 0,7, field 707×707 µm acquired at 1024×1024 pixels) to analyze the number of IGF-1 and NeuN positive cells in sections double labelled for IGF-1 and NeuN.

Next, we evaluated the differentiation of IhNSC-Ps into specific neuronal and glial phenotypes following transplantation into the ischemic environment by analyzing the colocalization of selective markers for neurons, astroglial and oligodendroglial cells with the anti-human specific antibody anti-huN.

Instead, we performed confocal microscopy to analyze the colocalization of ERGIC with ATG14 and DFCP1, two early autophagy markers as indicated in the manuscript.

Brain tissues were collected from the frontal lobe of hUCB-MSC-transplanted AD mice at 20 days and analyzed by using anti-human nuclei (green) and ICAM-1 (red) antibodies to analyze the colocalization of ICAM-1 and hUCB-MSC.

To analyze the colocalization of microglia and NEP expression in the hippocampal region of 20-day-old mice, double staining was performed using antibodies against NEP and CD11b in coronal brain sections.