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We also analyzed the cells for markers for photoreceptor differentiation at the end of 2 months of induction.
Forty-eight hours later, we analyzed the cells for NA expression level, hemadsorption and enzymatic activities.
We analyzed the cells for genetic polymorphisms by polymerase chain reaction in a PTC-200 thermal cycler (MJ Research, Watertown, MA, USA), as described previously (Hong et al. 2000).
To ascertain that the inhibition of FLT3 signaling is independent of the JAK2 activity of pacritinib, we treated the cells with the JAKi-1 (a selective pan JAKi) and analyzed the cells for pFLT3.
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To determine the mechanism for this anergy, we tested whether these cells were mycoplasma contaminated by analyzing the cells for mycoplasma DNA by PCR.
We assessed our ME2 knockdown cells for their degree of megakaryocytic differentiation by analyzing the cells for expression of CD10, a cell surface glycoprotein expressed selectively on mature megakaryocytic cells that is induced in K562 cells in response to PMA stimulation.
To adequately determine the effects of combination, the slices were harvested 24 h later (when the effects of single-agent AdsTRAIL were minimal) by preparing a single-cell suspension from the slices and analyzing the cells for the sub-G1 (apoptotic) fraction.
We also analyzed the cell lysates for the invariant surface glycoproteins ISG65 and ISG75, and observed increased levels of both after four hours MG-132 exposure.
To evaluate the level of WGA artifact mutations we created small colonies, each founded by a single RMA cell [18], and analyzed the cell identifier for each progeny cell (using the same set of MS loci).
We treated U266 and RPMI8226 multiple myeloma cells, HL-60 leukemia cells and human U2OS-C3 osteosarcellscells with proteasome inhibitors MG115, MG132 and bortezomib (Fig. 2A) and analyzed the cell lysates for the levels of FoxM1 by immunoblotting.
We analyzed the cell encapsulating droplets for cell viability and performed genomic analysis on the printed stem cells and compared them to the non-patterned controls.
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