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The correlation coefficient was ≥0.80 for all analyzed substances.
This is especially important either if comprehensive chemical analysis of eluates is not possible, or if the environmental impact of the analyzed substances is unknown.
Taking into account the low volatility of the analyzed substances, the temperature dependence of the saturated vapor pressure was determined by the integral Knudsen effusion method.
Hence, in this case the analyzed substances like PAHs and PCBs are strongly supposed to originate from the road traffic near the Argen River.
However, a clear link between the observed effects and the analyzed substances cannot be established, since the chemical analyses focused on substances which mainly represent priority substances.
A comparison of our results with the literature shows that the analyzed substances like PAHs and PBDEs probably contributed to the pathologic alterations in the investigated fish tissues.
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Detailed information on the analyzed substance groups and substances is provided in Appendix 1 (Table 4).
Sometimes the concentration of analyzed substance is too low, or dilution is undesirable for technical reasons.
The densitometric visualizing index (DVI) [ 44] was proposed and defined as (5) DVI = p 2 m 1 × α × 1 0 − 4 [ AU μ g · ° ], where m1 is the limit of detection of the analyzed substance [ μ g], p2 is the spot area [AU] of a μg of analyzed substance, and α is the angle between the tangents at the inflection points to the curves of the densitometric band of a μg of analyzed substance.
We analyzed substance-related behaviors potentially associated with binge drinking [ 16, 44], collecting information on habits of smoking nicotine cigarettes and/or cannabis during the last 30 days.
The broadening index [ 41, 42] was defined as (2) I broad = a p 2 × 1000 [ μ g AU ], where a is the mass [ μg] of the detected substance and p2 is the spot area [AU] of a μg of this analyzed substance.
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