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An analyzed spot size was 1 2 μm.
Table 2 shows protein scores and peptide counts for each analyzed spot.
The ratio of exposed and sham sample expression was analyzed spot by spot, after logarithm transformation, with variance analysis (anova).
Per analyzed spot, the PMF and a maximum of 10 MS/MS spectra were used for protein identification by Mascot search (V2.3, Matrix Science Ltd, London, UK) against the NCBInr database limited to the taxonomy of Arthropoda (2,341,519 sequences, download date 08/05/2014) and assembled mRNA sequences from I. ricinus midgut transcriptome sequencing (364,158 sequences).
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Red spots indicated by red arrows are analyzed spots.
Locations of these analyzed spots were chosen according to the main tones and the heterogeneities observed in the painting.
Here, the final conclusions, the produced spectra and a map of the analyzed spots are presented together with a commentary text.
We further analyzed spots 1 3 by scraping the cellulose extracting the peptides and subjecting them to the MS analysis.
In no case could a second protein be identified indicating that the analyzed spots did not contain major contaminating proteins.
The analyzed spotted fever group genomes form a monophyletic cluster of taxa with little sequence divergence relative to the other rickettsial groups (Figure 3).
For As91, 11 of the 19 analyzed spots were LMW-GS proteins, which matched separately to the deduced proteins of five LMW-GS genes (Figure S3A, Tables 2 and S8).
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