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However, the fraction of those nanoparticles in different aging states is difficult to be analyzed only by transmission electron microscopy (TEM) due to the limitation of two-dimensional observation.

In these studies, however, the total beta-catenin level was analyzed only by immunoblot analysis.

Perturbation experiments with H1a, H1c and H1° were analyzed only by SDS gel electrophoresis.

At present, the level of SMCs can be quantitatively analyzed only by penis biopsy and immunohistochemistry [ 5, 6].

Physical strength in PWS has been previously analyzed only by means of non-instrumental functional tests [ 6].

To date, synergistic interactions of plant HSFs that enhance transcriptional activation have been analyzed only by transient expression [ 15, 34, 35].

Secondly, the MRI results were analyzed only once and by only 1 observer, so the intra- and interobserver reliability remains unknown.

We did not observe any subchondral bone overgrowth or ectopic bone formation although we analyzed only 10 cases by radiographs and MR images.

As fourth limitation, we analyzed only hospitalization seen by infectious disease specialists and a generalization to all IVDU hospitalizations may be limited.

In order to exclude possible bias introduced by uneven distribution of CDSs in these genomes, we analyzed only the orthologs shared by three of these genomes, Mic-PCC7806, Syn-PCC6803 and Cwa-WH8501.

For the family-based data, we have analyzed only the trio families by MDR phenomics version 1.0 [ 43].