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Protein bands were identified using an ECL system (Millipore, Bedford, MA, USA) ATAD2 expression was analyzed on paraffin-embedded specimens from 129 patients.
To confirm that retinal lesions of neovascular retinopathy specimens exhibit Albumin hyperpermeability as previously reported (Vinores et al., 1989, 1990 , 1993 Knudsen et al., 2002), Albumin distribution of expression was analyzed on the same specimens.
All tumors classified as "good responders" to chemotherapy were analyzed on the pretreatment biopsy specimen.
The data comprised 505 ccRCCs and 70 normal kidney specimens analyzed on the Illumina HiSeq platform.
In this study, WT1 protein expression using IHC was analyzed on only malignant tumor specimens.
Specimens analyzed on batch dates with extremely high or low variability were re-analyzed, and the two values averaged.
The cell specimen was analyzed on the FACScan and antibody-binding capacity calculated by interpolation on the calibration curve.
A random blood and urine spot specimen was collected and analyzed on the same day in one central laboratory (no frozen samples).
Some specimens were analyzed on Shimadzu LCMS-2020 cofsisting of an automatic injector, a gradient pump, a diode array detector and a mass analyzer.
Specimens were analyzed on a Nikon Eclipse epifluorescence microscope equipped with a Hamamatsu CCD camera and data collected in Metamorph under non-saturating conditions (Molecular Devices).
Specimens were analyzed on an Olympus BX51 microscope and Photoshop 5.0 software (Adobe, San Jose, CA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com