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We obtained six different clones from this population, and they showed a quite variable relative expression in all analyzed markers, with no particular correlation with their differentiation ability.
Approximately 49% (314/639) of the analyzed markers detected polymorphism among the recurrent and donor parent.
However, almost all analyzed markers were developed by studying the sequences of the Nasonia genome assembly 1.0.
Chromosome anchored markers ([27], [28]; Table S3) revealed that five of the seven groups corresponded with the five chromosomes (Chr 1 5) of Nasonia and that these five groups comprised 99.9% of all analyzed markers.
An univariate analysis was performed for each clinical variable and for the tumor expression in tissue of the five analyzed markers (Ki67, CD20, bcl-2, c-kit and TiA1), first with continuous variable and then with binary variable.
This null distribution typically depends on the (usually unknown) demographic history of the populations surveyed and two main types of strategies have been reported to estimate it, using either i) data simulated under demographic models [6] which are generally simple and restrictive or ii) directly from the observed data under the assumption that most of the analyzed markers are neutral [7] [9].
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Actually, all these 4 tag SNPs were included in our analyzed marker list.
In order to obtain a comprehensive picture of the Iban population, we analyzed marker sets for autosomal, Y, and mitochondrial chromosomes.
WW isolated samples, generated the SSR and SNP markers, analyzed marker data, and wrote and revised the manuscript.
YW generated the InDel markers, analyzed marker data and drafted the manuscript.
Table 1 also indicates the numbers of samples available for each analyzed marker.
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