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The analyzed library was derived from a DNA pool of in vitro SCRaMbLEd pLM495 by linearizing with NotI and SalI.
The relative abundance of EST categories varied according to the analyzed library.
The number of sorted reads from each library mapped to each unigene (unigene-count) was used as the representation of that unigene in the analyzed library.
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TK, JC, ZT and JA analyzed libraries.
Therefore, we examined the origin of miR-92a elevated levels in the analyzed libraries.
In addition, the newly identified A. gambiae ortholog of mir-2940 also predominantly produced miR-2940* miR-2940*l acrossed libralles (Tanalyzed.
Furthermore, we observed a bias toward uridylation in miRNA sequence reads (around 81-84%) derived from the 3'-arm of the precursor miRNA across analyzed libraries.
The expression level of several miRNA varied between the six analyzed libraries, indicating a differential functional role of the involved genes in the development-associated regulation.
Highly expressed Anopheles miRNAs (e.g. bantam or miR-263a) are represented by numerous mature sequence variants that vary in their abundance across all analyzed libraries.
Among them, hsa-mir-500a, hsa-mir-548h-3, hsa-mir-664 and hsa-mir-1307 hsa-mir-1307 hsa-mir-1307t wereh the major-minor switch was obsextremen all of the analyzed libraries, incasesng three normat and twhichVHCC libraries.
3- to 10-fold; group III elements, <3-fold; and group IV elements were expressed below an RPKM cutoff of five in any of the analyzed libraries (no or very low expression and not further analyzed).
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