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One sample was prepared from each line and analyzed in triplicate to determine the concentrations of NA and DMA.
All skin biopsies were analyzed in triplicate to minimize random variation.
Each disk included a pool of total RNA from bovine liver samples analyzed in triplicate to be used as the basis for the comparative expression results (exogenous control) and duplicate tubes of water (non-template control).
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The samples were analyzed in triplicates, to obtain the optimum result of 10 40 μg L−1.
The soil was analyzed in triplicates to determine its initial physicochemical properties including texture, pH in water (pH-H2O), electrical conductivity (EC), organic matter, available nitrogen, available phosphorus and total exchangeable bases and acidity.
Samples were analyzed in triplicates to reduce the manual error while handling the samples.
Each cryogel sample was analyzed in triplicates to reduce the error.
The chemical composition of the hull (Table 1) was analyzed in triplicate according to the following methods.
All of the samples were analyzed in triplicate in order to make the data reliable for statistical analysis and to increase protein coverage using stringent protein identification criteria.
The reference materials were dissolved and analyzed in triplicate, whereby according to our internal specification for quality control, the recovery rate for aluminum had to be between 90 and 110%.
The data of the various parameters of compost maturity and enzyme activities were analyzed in triplicate and subjected to ANOVA (analysis of variance) in accordance with the experimental design (completely randomized block design) using SPSS-16 statistical package to quantify and evaluate the source of variation and CD (critical difference).
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