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To determine the limit of quantification (LOD), serial dilutions of yellow fever virus (10 to 6.25 RNA copies/reaction) were made and eight independent replicates were analyzed in the same assay.
Statistically significant results can be obtained if the experimental design includes a sufficient number of replicate 2-DE gels, and if the replicate gels are similar enough to be analyzed in the same experiment.
A sample was considered positive if the average absorbance value of the three replicate wells was four times greater than the average absorbance value of healthy uninoculated samples of non-transgenic plants analyzed in the same plate [ 39].
However, less than 50 replicated SNPs were analyzed in the review article by Iles.
For precision and accuracy, QC samples at four different concentration levels (LOQ QC, LQC, MQC and HQC) were analyzed in six replicates on the same day (intra-day) and on three different days (inter-day) respectively.
Irrespective of their origin, three replicates were used for all samples analyzed in the present study.
Each bootstrap replicate was analyzed under the same conditions as the starting dataset but using only 2.5 × 105 generations (a burn-in of 50000 generations was used).
To determine the accuracy and intraday precision, pure LOS solutions of three different concentrations were analyzed in six replicates each during the same day.
Coefficient of variation was calculated for replicates analyzed by the same system (intra-laboratory) and across different laboratories (inter-laboratory) [125].
The technical replicates were all from the same purification, and they were analyzed on the same day on the same machine, consecutively.
Samples were analyzed in triplicate as biological replicate or quadruplicate as technical replicate.
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