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Finally, we employed a derivatization approach where the PtdEtn molecular species were tagged by addition of Fmoc and analyzed in the negative-ion mode (Figure 6D) as previously described [5], [16].
Samples were analyzed in the negative-ion mode.
RNA sequences were analyzed in the negative-ion mode with a potential of −4 kV applied to the spray needle.
RNA-amino acid conjugates were analyzed in the negative-ion mode with a potential of −4 kV applied to the spray needle.
RNA-peptide conjugates were analyzed in the negative-ion mode with a potential of -4 kV applied to the spray needle.
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Compounds were analyzed in the negative ion mode.
Therefore, this sample has also been analyzed in the negative ion mode and indeed more sialylated-, O-sulfated-glycans, and glycans having reducing end aldohexonic acid residue were observed due to the improved sensitivity for the detection of negatively charged molecules (data not shown).
The recovered lyso-aPtdEtn molecular species with the lyso-dPtdEtn internal standard were analyzed by ESI/MS in the negative-ion mode.
The ether lysoPtdEtn molecular species were analyzed by ESI/MS in the negative-ion mode and the presence of very long aliphatic chains containing high degrees of unsaturation in low abundance (e.g., the ion at m/z 510.2) was detected (Figure 5A).
For example, PtdEtn molecular species from mouse cerebellum were analyzed by survey scanning in the negative-ion mode using an LTQ-Orbitrap mass spectrometer after direct infusion of diluted lipid extract solutions supplemented with a small amount LiOH (similar to Figure 6A).
Samplesentary data 1 H and C NMR spectra for compounds 2 – 7.
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