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Each patient's RNA sample (PT1, PT2, PT3, PT4) was co-hybridized with a control's pooled RNA (C1+C2+C3) and analyzed in replicate with dye swap, for a total of 8 slides (n = 8).
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In order to estimate genotyping concordance 16 Lvg individuals from the Gracen population were analyzed in replicates.
Biological replicates were analyzed in triplicate with gene expression normalized to β-actin and fold change determined using the ΔΔC t method.
Purified peptides were analyzed by reverse-phase liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and each sample was analyzed in technical replicate [ 35].
Each sample was analyzed in four replicates, and the qRT-PCR data were analyzed with LightCycler® 480 software version 1.5 (Roche).
Serum samples with 5, 10, and 20 ng/mL HMGB1 protein concentrations were analyzed in 10 replicates for assessment of intra-assay precision.
Each aptamer concentration was analyzed in three replicates.
Each sample collected in the experiment was analyzed in three replicates.
Fat acidity (KOH mg/100 g) was analyzed in three replicates by the AOAC ([1970]) method.
All samples were analyzed in four replicates.
Each gene was analyzed in three replicates.
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