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For quantification, Western blot films were scanned and analyzed in Image J (NIH).
Cell numbers were analyzed in Image J by manual markup of individual cells in the stacks (Cell Counter plugin).
The images were converted to black and white in image processing software Picasa version 3.9.16.37 and analyzed in image processing software ImageJ version 1.47 (Abramoff et al. 2004).
To calculate the rate of ROS production in neurons cultured under various conditions, Dhr123 time series were analyzed in Image J.
Synaptic connectivity was analyzed in image stacks with voxel size 0.103 μm (x/y-axis)–0.3 μm (z-axis), whereas neurite territories were measured in image stacks with voxel size 0.206 μm (x/y-axis)–0.5 μm (z-axis).
Images were taken of midguts approximately 300 μm from the anterior pylorus in the 568 nm channel using the Zeiss 780 combined confocal/FCS/NLO system and analyzed in Image J. Mean pixel intensities for J-aggregates were averaged, and significant differences between means were determined with a Mann-Whitney U test.
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Cortica, a startup developing technology for analyzing in-image content, is announcing that it has raised $6.4 million in Series B funding.
In the segmentation phase, we evaluate the difference between the texture synthesized thanks to the model and the texture analyzed in the image (Figure 3(f)).
The images were analyzed in Duolink image tool (Sigma-Aldrich, Germany), the drawing tool was used to exclude background signals on the slide and partial cells.
In total, 1,536 features (half stromal, half epithelial) were analyzed in each image from TMA cohort 1 (464 TMA images from 232 patients).
These are then analyzed in Scion Image with the help of the macros for obtaining nuclear data.
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