To confirm the results obtained with the cell line models (see above) in laforin knockout (Epm2a-/) mice, several markers of ER-stress were analyzed in extracts from different tissues of 9-month old mice.
The compounds, gallic acid, ellagic acid, corilagin, geraniin, phyllanthin and hypophyllanthin were identified and quantitatively analyzed in the extracts of Phyllanthus amarus and P. urinaria obtained from Malaysia and Indonesia by using a validated reversed phase high performance liquid chromatography (RP-HPLC) method.
Based on the method described by Allen [19] and Harbone [20] the following major phytochemicals such as alkaloids, tannins, saponins, terpenoids, phenols, flavonoids, coumarin, glycosides, reducing sugar, steroids and resins were spectrophotometrically analyzed in ethanol extract of T. conoides.
The TPH1 protein was analyzed by western blot in extracts of these cells and was significantly elevated not only in MDA-MB-231 and T47D, but also in MCF7 cells, demonstrating that 5-HT biosynthetic capacity was increased in all breast cancer cell lines tested, with the highest level of expression occurring in MDA-MB-231 cells.
Finally, for the focus group, digital recordings were analyzed in order to extract the number of times in which psychosocial difficulties and related variables had been a topic during the session (i.e., number of times that these issues had been repeated by a different participant).
Total phenol concentration was analyzed in the aqueous extract of compost according to the method of Bray and Thorpe (1954) and expressed in mg/g of dry compost.
We subsequently found that expression of HSP60, a mitochondrial matrix chaperone, was significantly elevated in protein extracts analyzed in a subset of responder PBMCs, compared to non-responders (at day 7 post-vaccination in young subjects and on all days in older subjects).
Three independent quality-control samples in plasma (2.89, 40.7, and 571 ng/mL) were prepared fresh and analyzed in triplicate in each batch of extracted plasma samples.
The metabolites in extracts of medium were analyzed by HPLC to investigate the metabolic capacity of bovine liver slices.
To determine whether signaling through ER and/or PR were involved in modulating MMP activities, we analyzed tissue extracts of ovariectomized mice exposed to exogenous estrogen and/or progesterone.
In the present study, we analyzed the extracts of 556 blood samples from 298 mice from the nonvaccine control grids by a multiplex, quantitative real-time PCR for 16S rDNA that discriminated between B. burgdorferi and B. miyamotoi at the site (4).
