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Fasting plasma glucose levels were analyzed in duplicate with a glucose oxidase analyzer (Yellow Springs, OH, USA).
Three working standards calibrated to VSMOW and standard reference waters supplied by the International Atomic Energy Agency IAEAA) were analyzed in duplicate with each batch of water samples analyzed.
Serum samples were analyzed in duplicate with the antigen and once without antigen (coating buffer only).
Each sample was analyzed in duplicate with the volume of a single reaction added up to 15 µl which contained 20 ng template and a final primer concentration of 0.6 µM.
The esterase activities in the supernatant of the fungal cultivations were assayed using the above substrates and the release of the acids measured by HPLC as previously described [32], except that incubations were performed at 40°C for 60 min. All assays were prepared and analyzed in duplicate, with 10% standard error for each set of results.
Each sample was analyzed in duplicate with an ABI 7900-HT (Applied Biosystems, Foster City, CA) as follows: 1 cycle of 50°C/2 mins, 95°C/2 mins, followed by 40 cycles of 95°C/15 seconds, 55°C/30 seconds and 72°C/30 seconds, followed by a melt-curve analysis.
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Serum samples were analyzed in duplicates with Luminex Plate in a 1 2 dilution and per manufacturer´s directions.
All samples were analyzed in duplicates with all 4 sets of primers including a non-template control for each run.
All specimens were analyzed in duplicate (independent aliquots), with three furnace injections per analysis.
To ascertain analytic quality all standards, controls and samples were analyzed in duplicate and all duplicates with a coefficient of variation >10% were reanalyzed.
Additionally, one randomly selected sample was analyzed in duplicate in each batch with a coefficient of variation of 4.37% and 0.45% for DDE and p,p′-DDT, respectively.
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