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Preoperative and postoperative radiographs were retrospectively analyzed in a serial fashion by two independent observers (ESM and MSK) to evaluate the fracture type according to Neer's classification, bony union, humeral neck-shaft angle, and complications.
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Quality control was performed on each plate with a serum pool consisting of 4 serum samples that had been analyzed in a 1 4 serial dilution, starting with a dilution of 1 100 up to 1 409,600.
In order to evaluate the neutralization activity of larva-derived VHHs, recombinant single-domain antibodies were analyzed in a VN test using serial four-fold dilutions of antibodies and the Wa human RV strain (SbI, P[ 8]G1).
The data were labeled with serial numbers and analyzed in a way to protect patient privacy.
Areas of col IV deposition and of loss of astrocytic markers in the EAE and LPS models were analyzed in serial sections, using a microscopic field of 1 mm.
The NGF concentrations were analyzed in duplicate at serial dilution and were read against a standard curve.
After 60 min coagulation at room temperature serum was harvested by centrifugation and analyzed in serial dilutions for the presence of HEL-specific Ig by ELISA.
In addition, plaque size and composition were analyzed in serial longitudinal cryosections of aortic roots as described [ 34, 35].
Dendritic segments indicated by red circles and numbers and the somatic region (inset grey drawing) were selected and analyzed in serial ultrathin sections at the EM level.
To assess whether exogenous p190B expression affects metastasis the histopathology and number of metastases were analyzed in serial sections of lungs from tumor-bearing mice.
For visualization of the bound antibodies, diaminobenzidine was used as chromogen as described previously [ 18].> Areas of collagen IV (col IV) deposition and of loss of astrocytic markers in the EAE and LPS models were analyzed in serial sections.
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