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To further study the degree of sensitivity or resistance of each deletion strain in our phenotypic screen, the IC50 for each deletion strain was analyzed in a secondary validation screen and the target strains were arranged based on their degree of sensitivity to NiSO4.
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Andersen, T. E. et al. Escherichia coli uropathogenesis in vitro: invasion, cellular escape, and secondary infection analyzed in a human bladder cell infection model.
The patient-reported perceived deficits questionnaire (PDQ) and physician-assessed clinical global impression (CGI) were analyzed in a prespecified hierarchical testing sequence as key secondary end points.
Secondary endpoints were analyzed in a similar fashion.
Secondary outcomes will be analyzed in a similar fashion.
HUVEC and EAhy926 cells were suspended in PBS, incubated with anti-ATP synthase mAb and FITC-conjugated secondary Abs, washed and analyzed in an Epics XL (Beckman Coulter) flow cytometer.
All secondary objectives will be analyzed in an exploratory manner.
The secondary outcomes will be analyzed in an explorative, descriptive manner.
Secondary endpoints will be analyzed in an exploratory fashion, using appropriate statistical methods based on the underlying distribution of the data.
Results from the waiting controls after their 12 weeks of exercise game intervention were analyzed separately in a secondary analysis.
Indeed, our results emphasize this important fact, since not only did we find differences in performance between various wild type genetic backgrounds, we also saw dramatic phenotypic differences in the mutant strains carrying the same mutation but in a different genetic background as analyzed in detailed secondary screens.
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