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The genotype of the vegfc hu6410 zebrafish mutants was analyzed in a multiplex PCR with WT and mutant zebrafish-specific inner and outer primers.
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As a panel of seven markers can be analyzed in a single multiplex, the cost for genotyping seven markers may not be very much different from the cost for a one-marker analysis.
Interleukin-6 (IL-6), interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) were measured by a multiplex cytometric bead-based assay (FlowCytomix Multiplex; BenderMedsystems, Vienna, Austria) and were analyzed in an EPICS-XL-MCL flow cytometer (Beckman-Coulter, Fullerton, CA) following the manufacturer's protocol.
To further investigate the linkage in the presumptive candidate region, microsatellite markers in a 2 cM grid covering the interval from 164 to 203 cM were analyzed in 110 multiplex (2 or more sampled autism patients) families.
Eight VNTR loci were selected and analyzed in 2 multiplex PCRs.
Eight loci were analyzed in three multiplex reactions (Table S1) using the QIAGEN Multiplex PCR Kit QIAGEN Inc ... Samples were amplified with the following profile: initial denaturation at 94°C for 10 m, followed by 45 cycles of (94°C for 60 s, 58°C for 60 s, 72°C for 60 s).
Porcine cytokines (n = 9) were analyzed in plasma using a multiplex kit from Bio-Rad Laccordinges, Inc. according to the manufacturer's instructions.
"Rare" must be examined in light of the ability to analyze genes in a multiplex fashion.
Levels of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in blood serum were analyzed using a multiplex immunoassay on day 28 after immunization with CIA.
Isolates in Year 1 were analyzed using a multiplex rapid-cycle polymerase chain reaction (PCR) with microwell-format probe hybridization, as described previously [ 28]; a Taqman®-based PCR assay was used in Years 2 4 [ 29].
Nine proteins were analyzed with a multiplex selected reaction monitoring assay in an independent set of Trisomy 21-amniocyte samples and two of them (SOD1 and NES) showed a consistent differential expression.
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