Crops and foods improved through biotechnology are routinely analyzed for their environmental performance and food safety.
Micrographs were analyzed for the quantity of synapses and for synapse abnormalities (reduction or changes in synapse and dendritic filopidal spine morphology, i.e., degradation of cytoskeletal architecture).
The resulting rate function α l (c) or equivalently j l (c) = cα l (c) can be used for microscopy data: cell images are analyzed for the quantity K and for fluorescence intensities which allow calculating the protein concentrations of interest.
Total RNA from U2OS, MG63 cells and osteoblasts was extracted using the TRIzol reagent according to the manufacturer's instructions (Invitrogen), and analyzed for quantity and quality using bio-analyzer (Agilent Technologies, USA).
With the 2100 Expert® software, the isolated RNA was analyzed for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, using the RNA 6000 Pico Series II assay).
The RNA was resuspended in 50 µL DEPC water, cleaned by passing over silica-based mini-spin columns (Qiagen RNeasy PlusMini Kit, Valencia, CA, USA) and analyzed for quality and quantity on a 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA) and concentration was adjusted to 1 µg/µl.
The RNA was analyzed for quantity and quality on the Bioanalyzer (Agilent) and a standard spectrophotometer (Agilent).
Total RNA extracts were immediately analyzed for quantity (OD260nm) and purity (OD260 nm/OD280 NanoDropoDrop ND-2000 c, Peqlab GmbH, Erlangen, Germany).
Amplified RNA was analyzed for quantity and quality on the Bioanalyzer 2100 (Agilent) using the Agilent Total RNA Nano Chip assay.
