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Forty-two single-site alanine substitutions of the 2C TCR were analyzed for binding to QL9/Ld and anti-TCR antibodies.
Furthermore, full-length and mutant HcpA proteins were analyzed for binding of human PLG using ELISA.
For the first round of sorting, approximately 1×108 yeast clones were analyzed for binding to 100 nM αvβ3 integrin.
When an unbiased set of 15 lectins was analyzed for binding to six B-cell lines, 12 of the lectins showed significant staining.
After incubation, the non-bound Salmonella was thoroughly washed off, after which the cells were fixated with 2% paraformaldehyde in PBS and analyzed for binding of FITC-labeled Salmonella by flow cytometry using the FACScalibur (BD Biosciences).
A panel of small pox peptides representing good binders of KD<10 nM (YLDYDTIYV, FLRDNLYHV, and YLSDSAINI), intermediate binders of KD 10-100 nM (YLSTERDHV and FLETDAGRV), and non binding peptides of KD>1 µM (ALSDACKKI), were analyzed for binding affinity to each of HLA-A*0201 HLA-A*0201cts.
Similar(43)
Highly similar putative CTL epitopes were analyzed for their binding with the HLA-C 12*03 molecule.
Sections were processed and analyzed for [3H]glutamate binding to NMDA receptors using receptor autoradiography and for mRNA for the ζ1 (NR1), ɛ1 (NR2and and ɛ2 (NR2B) subunits of the NMDA receptor using in situ hybridization.
The most active compound d4 was analyzed for heme binding activity using UV spectrophotometer.
The best conformations of the ligands were analyzed for their binding interactions [40].
The diazepine derivatives derived in the present study were analyzed for the binding affinity with NS5B polymerase.
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