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FRα levels in serum were analyzed by two methods, immunoblotting analysis and a radiolabeled folic acid-based microfiltration binding assay.
Typical recordings of control and TM23 mutant hearts are shown in Fig. 3. To take this kind of pausing into consideration, heart beat in both control and TM23 mutant larvae was analyzed by two methods, counting the number of heart beats over either periods of 10 seconds or 1 minute.
A sample of the Arcobacter butzleri type strain ATCC 49616 was analyzed by two methods: 1) an Arcobacter multiplex speciation PCR [134] and 2) amplification and sequencing of the 16S rDNA locus, using the primers 16ARCO-1 (5' ATG ATGACAC GAA AGT CTG AT 3') and 16ARCO-2 (5' CGC AAT CGG TAT TCC TTC 3').
The relation between the hospitals' strategy and its outcomes was analyzed by two methods.
The tissues were digested in filtered sodium hypochlorite, and the residual dust was analyzed by two methods.
In-silico enrichment of ChIP-Seq binding was analyzed by two methods; a one-tailed Fisher's exact test and a resampling based test examining both the number of bound promoters as well as the total number of identified ChIP-Seq peaks in a gene list compared to 100 000 randomly sampled gene lists of equal size.
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The effect of build orientation on cylindricity error is analyzed by three methods: first by a simple analytic method, second by simulating the manufactured surface using a CAD (Computer Aided Design) file of the part and third by using an STL (Stereolithography) file.
Most of these individuals were analyzed by three methods: MAPH, REDVR and PRT as described previously in great detail [16], [23], [35].
Apoptosis was analyzed by three methods: 1) Flow cytometry: Apoptotic cells were analyzed using the Annexin-V-FITC/PI kit (BD, San Diego, USA) by a FACSCalibur flow cytometer (BD) according to the manufacturer's instructions.
The aCGH data of the 83 gastric cancers were analyzed by two independent methods referred to as method 1 and method 2 for convenience.
The deduced TFAs were then analyzed by two complementary methods.
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