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The VHH DNA repertoire was analyzed by sequencing.
Colonies were isolated and candidate genes in the plasmids that conferred cysteine resistance were analyzed by sequencing.
Plasmid DNA was isolated from individual clones, purified, and analyzed by sequencing.
Plasmid DNA is prepared from resulting colonies and analyzed by sequencing.
A- and B-type RIFIN RT-PCR products from each developmental stage were cloned and single colonies were analyzed by sequencing.
In both cases the E1-E2 regiof of the HCV genome was analyzed by sequencing viral clones and the former study also included clones from the NS5A region.
The PCR products from this reaction were analyzed by gel electrophoresis, and predominant products were purified, cloned into PCR2.1 and analyzed by sequencing.
PCR amplicons were gel-purified, subcloned into pCR4-TOPO vector, purified in 96 well plates (Qiagen Turbo Miniprep) and for each subject, 12 clones from each immunoprecipitate were analyzed by sequencing.
Similar(3)
All MS/MS data was analyzed by sequence database searching using the program Sequest [86] against protein sequences derived from all known open reading frames in S. cerevisiae.
HLA class II was analyzed by sequence-specific primed PCR.
The similarity of these sequences was analyzed by sequence alignment software (Clustal).
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