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Figure 2 clearly shows for both genetic backgrounds that the recombinant strains do produce low, but significant and reproducible amounts of vitamin C. Wild type cells as well as recombinant cells transformed with ScALO and AtLGDH were analyzed as negative control.
The same genes were not expressed in fresh amniotic fluid samples, analyzed as negative control (not showed).
In each assay negative controls containing pure water as well as pure SF in the spiking assays were analyzed as negative controls.
The number of sequences analyzed as negative control was 99,759 and of these only 317 were predicted as containing IRESs (false positives).
GFP-immunoprecipitations from 2 batches of asynchronized 3D7-HT-GFP cells and immunoprecipitations in the absence of an antibody from 2 batches of asynchronized GFP-PfATG8 3D7 cells were also analyzed as negative controls.
Furthermore, the amplified 16S rDNAs of two additional reference strains (Bacillus cereus ATCC 14893 and Staphylococcus aureus ATCC 9144) were analyzed as negative controls, resulting in no fluorescence signal against any of the tested probes.
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(The subtraction sign counts as one negative).
welwitschiae from the other A. niger aggregate species analyzed as a negative control.
Ovaries from the M line Cantony were also analyzed as a negative control.
Human ADSCs were also analyzed as a negative control.
Sections incubated without the p-CREB primary antibodies were analyzed as the negative controls.
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