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The fluorescence data was collected and analyzed as indicated.
The positive samples were selected and the corresponding wings were analyzed as indicated above.
The data are presented as the means and standard deviation (SD), or standard error of the means (SEM) where n represents the number of pseudopodia or number of cells analyzed, as indicated.
For Cyclin D1 expression, cells were extracted in RIPA Buffer (1% Triton X-100, 0.1% SDS, 1% Na-deoxycholate, 150 mM NaCl, 50 mM Tris-HCl pH 7, 0.4 mM Na3VO4, 10 µg/ml leupeptin, 4 µg/ml pepstatin and 0.1 Unit/ml aprotinin) and analyzed as indicated above.
Growth rate was analyzed as indicated [ 15].
Total and phosphorylated proteins were analyzed as indicated.
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Finally, most genes residing on the genomic region comprised in the BAC clone HM061166.1, thus surrounding the HAPPY locus, were expressed in the different flower parts as well as in both sexual and apomictic flowers analyzed in this study, as indicated by the production of multiple sequence reads matching the CDS predicted from the genomic sequence HM061166.1 (see Additional file 1: Table S4).
For the bark library, 11,611 sequences were collected from the dbEST database; the sources of the other libraries analyzed were as indicated in Table 1 (i.e. the male bud library was sourced from CMFL, the female bud library from CFFL, the leaf library from CLFL, and the seedling library from TUM25).
The variation of Inormalized for the entire tumor was analyzed, as shown in Figure 4b, indicating that Inormalized for the entire tumor around the first day reached the minimum for mouse 2. However, for mouse 1 at the 18th and 30th hours, close to the first day, Inormalized for the entire tumor had no significant difference from at the 0th hour.
All data were dichotomized as indicated in the tables and analyzed as categorical variables.
Therefore, when a stretch of text is identified as containing a coherent rhetorical function, it will be analyzed as a move whose labeling unambiguously indicates its function.
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