Sentence examples for analyze the splicing patterns from inspiring English sources

To analyze the splicing patterns, 5 µg of total RNA were treated with DNase I and reverse transcribed (RT) with SuperScriptII RNase H− RT following instructions from the provider (Invitrogen).

In order to quantitatively analyze the splicing pattern of an exon pair, we separately aligned each exon against the human EST database using BLAT [42].

Therefore, we also analyzed the splicing patterns of Lef1 and Tcf7l2 in the developing and adult brain.

We analyzed the splicing patterns of the 11 769 cDNAs by using the 5'-EST sequence data (Fig.  2).

To verify whether the splicing reprogramming resulting from lamin B1 dysregulation is mediated by raver-2, we analyzed the splicing pattern of PTB-target genes in MEFs.

The obtained SJ set, named TopHat_Rev set, was used to analyze the splice site sequences and alternative splicing.

Further, the observed differences in the splicing patterns among the analyzed genotypes were probably diluted by the effects of common tester alleles.

Comparing with a benchmarked exon array data that were also analyzed by the "Splicing Index" algorithm, we found that the proportions of the splicing patterns observed in HUVECs treated with CoCl2 are different from those in the benchmark dataset [ 26].

The splicing patterns of several other genes coding for serine proteases such as trypsins, chymotrypsins, CFD, and plasminogens (PLG) [49] and KLK-like toxin [48] were also analyzed.

The construct is then transiently transfected into eukaryotic cells and the splicing pattern is analyzed by RT-PCR with specific primers of plasmid exons.

We used the GeneChip® Human Transcriptome Array 2.0 (HTA 2.0) for analyzing differential gene expression studies to analyze differential splicing patterns in NT and iAs-T cells.