Sentence examples for analyze the splicing pattern from inspiring English sources

In order to quantitatively analyze the splicing pattern of an exon pair, we separately aligned each exon against the human EST database using BLAT [42].

To analyze the splicing patterns, 5 µg of total RNA were treated with DNase I and reverse transcribed (RT) with SuperScriptII RNase H− RT following instructions from the provider (Invitrogen).

To verify whether the splicing reprogramming resulting from lamin B1 dysregulation is mediated by raver-2, we analyzed the splicing pattern of PTB-target genes in MEFs.

Therefore, we also analyzed the splicing patterns of Lef1 and Tcf7l2 in the developing and adult brain.

We analyzed the splicing patterns of the 11 769 cDNAs by using the 5'-EST sequence data (Fig.  2).

The obtained SJ set, named TopHat_Rev set, was used to analyze the splice site sequences and alternative splicing.

The construct is then transiently transfected into eukaryotic cells and the splicing pattern is analyzed by RT-PCR with specific primers of plasmid exons.

As an independent test of a change in the splicing pattern, the same RNAs used in the Northern blot experiments were analyzed by qRT-PCR.

We conclude that NPAP1L is expressed in the cow, primarily in the brain, but that the splicing pattern predicted by Ensembl is not correct in any of the analyzed tissues.

Snail1 also alters the splicing pattern of HNRNPH3, another protein involved in the splicing process [24].

The exon table, the splicing pattern table and the splicing event table are then generated, for each alternatively spliced gene.