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Histological analysis was performed by three individual pathologists to analyze the section.
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Antibodies against 13 proteins were used to analyze the sections (Table 1).
To determine how tendon tissues would alter after long-term hyperglycemia, we dissected the Achilles tendons from mice with type 1 DM, the duration of which was at least 1 year, and analyzed the sections using histochemical staining methods.
We analyzed the sections further by staining for Pdgfr-α.
A pathologist who worked blindly analyzed the sections using Eclipse 55i microscope equipped with a DS-L1 camera (Nikon, Tokyo, Japan).
We also observed these tangle-like and thread-like staining patterns when analyzing the sections for aberrant tau, UBB+1, and VDAC1.
In this section, we qualitatively analyze the cross section of a laser-induced BSF.
The framework approach was used to analyze the qualitative section's data.
Digital images were used to analyze the histological sections using ImageJ (NIH).
We used a MALDI-TOF-TOF-type instrument (ultraflex II TOF/TOF, Bruker Daltonics) to analyze the tissue sections.
To analyze the cross-sections of MAH-fed animals, worms were seeded onto plates and fed bacteria as described above.
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