In order to study whether endogenous neurexins are processed by a PS/γ-secretase-dependent activity, we first analyzed the processing of neurexins in cultured hippocampal neurons treated with vehicle or DAPT.
We then analyzed the processing of LC3-I protein to LC3-II, which is a hallmark of autophagy, using western blot analysis.
Finally, we also analyzed the effects of p38MAPK inhibition on cell death in the kidney by analyzing the processing of inactive caspase-3 in total kidney lysates and by TUNEL staining.
We tested calpain activity and calpeptin efficacy by analyzing the processing of a well-characterized calpain substrate, FL brain spectrin (240 kDa), which produces breakdown products of 150 and 145 kDa.
Overall, a similar trend was seen when analyzing the processing of 23S rRNA in the pre-30S, pre-50S, 50S, and 70S regions of the gradient; increasing sedimentation rate correlated with a decreased proportion of immature 23S rRNA relative to total 23S rRNA.
In this section, we analyze the processing times of the proposed heterogeneous implementation discussed in Section 4. The experimental tests as well as the evaluation of processing times were based on our methodology discussed in Section 3. First, all processing times reported in our work as well as the localization results are obtained using a real dataset (Section 3.1).
Through analyzing the processing and error sources of the method, the paper tries to demonstrate the feasibility and practicality of the method.
To analyze whether the processing of HA-βNrx1 required the activity of metalloproteases and PS/γ-secretase, we performed biochemical analysis in transfected cultures treated with GM6001, a broad metalloprotease inhibitor, or DAPT, a specific γ-secretase inhibitor.
Another area of neurolinguistics literature involves the use of electrophysiological techniques to analyze the rapid processing of language in time.
