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But these require dissecting the cells, so cannot analyze living, circulating cell populations.
The increasing number of available fungal genome sequences, knockout mutants, versatile tools for protein tagging, and the continuous improvement of fluorescence microscopes have allowed scientists to analyze living cells and reveal the molecular and cellular basis of septation with unprecedented detail.
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However, both sides also tend to focus on a single research paradigm, which analyzes living things in terms of certain macromolecular components.
Focusing on two relevant analytical methods for live-cell monitoring, we discuss the current challenges of analyzing living 3D samples: microscopy, which is the most widely used technology to observe and examine cell cultures, has been successfully adapted for 3D samples by recording of so-called "z-stacks".
It analyzed living conditions according to 39 factors, grouped in 10 categories that cover everything from personal freedom, availability of international schools and recreation; to access to consumer goods.
These physical quantities did not differ between nearby cells even if the expression levels were variable, suggesting that the tagRFP-5AA-eGFP construct had a homogeneous distribution within the sets of analyzed living HeLa cells.
Family is a dummy variable for family structure: living alone was expressed as 1 (other = 0), when the response to Scenario 1 was analyzed; living with children aged 5 years or younger was expressed as 1 (other = 0) when the response to Scenario 2 was analyzed; living with a relative aged 65 years or older was expressed as 1 (other = 0) when the response to Scenario 3 was analyzed.
When we analyzed living arrangements, households with only elderly persons had higher percentages for acute morbidity (21.6%), ambulatory care (17.6%) and hospitalization (6.3%) compared with the extended or complex households (households with elderly and other members or households without elderly).
Here, we present a protocol to execute and analyze live-cell microscopy experiments using KTRs.
We will analyze live performance, adapting techniques applied to written texts to space- and time-based events.
We have generated transgenic mice expressing GFP in their lymphatic vessels to analyze live lymphatic vessel development in normal and mutant animals by multiphoton imaging.
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