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These equations are solved numerically to analyze cycle parameters and to determine the amount of fresh water production.
Moreover, to analyze cycle phase dependent variations we measured all subjects twice.
Geometric means were generated to analyze cycle threshold (Ct) values; averages were generated to analyze copy numbers and mass values.
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On the basis of total counts for the analyzed cycles, we achieved precision of 2 3% for 13C14N and 1 4% for 15N12C, depending on enrichment and hybridization intensity (see Supplementary Information for additional NanoSIMS analysis details).
This was confirmed when analyzing cycles 2 6 as well as cycles 3 6 (Fig. 3).
Between cows of groups H and M, there were no differences in P4 concentrations of all three analyzed cycles (p ≥ 0.35; Table 1).
Our observations have shown that in the early stages of the analyzed cycles, the U6 snRNA was detected in the nucleoli at the same time that the Sm-containing CsBs appeared.
Separately analyzing cycles based on long or short intervals, we found that the peak of lumbar output in our preparations preceded peak cervical output by ≥150 ms in 25.9 ± 6.6% of respiratory bursts (arithmetic mean) (n = 21, Figure 6B,C,G,H).
FITC-BrdU kit (BD Pharmingen, San Diego, CA, USA) was also used to analyze cell cycle progression as previously described.
This method will be used to analyze the cycle, to determine which components need to be optimized.
A theoretical model is set up and then a numerical program is developed to analyze the cycle performance.
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